FastQCFastQC Report
Thu 26 May 2016
SRR1781333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608219
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35953659.112918208737305No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG43480.7148740831838531No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10790.17740320509553303No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7980.13120274111791969No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6990.11492570932509506No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6490.10670498619740588No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG150.002218791570.04455650
TGTTCGA150.002219516370.0387949
CGGTATC150.002219516370.0387955
TAATCGT150.002220241470.0330257
ATCGTAC150.002220966670.0272559
AATCGTA150.002220966670.0272558
CTGGCGA252.8522736E-669.94662518
ACGTCGG301.0300755E-769.935138
TACGTTG150.002232593369.9351310
TACGCCT150.002233321369.9293755
GTACGCG352.9961484E-759.9345364
CGTCTGG2950.058.0817159
TCTGGCG4000.056.8222911
CGCGTCT2100.056.6141477
TTGTGCG1800.056.3458982
GGATTGT252.4020886E-455.94356
CTGGCGC4000.054.19972212
CGCCGTA8600.053.7551253
GCCGTAT8700.053.53980354
TGTGCGC1250.053.1419563
Produced by FastQC (version 0.11.5)