FastQCFastQC Report
Thu 26 May 2016
SRR1781333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608219
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT19305031.740211996008018No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT498088.189155550878878No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG377626.208618934955994No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC145802.397162864034172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69211.1379124953347397No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC55540.9131579250237168No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT48210.7926421239717931No Hit
CAAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT40810.6709754216819929No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT33040.5432253842777026RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC24450.40199336094400206No Hit
CACTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC19770.32504739246883113No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC16430.2701329619758672No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG15710.2582951206719948No Hit
TCATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG12770.20995726868118228No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG11730.19285816457558874No Hit
CATAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT10980.18052707988405492No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT10860.1785541063334095No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT10540.17329284353168842No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT10120.1663874361044295No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC8140.13383337251878022No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC7480.12298201799023051No Hit
ACTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG6800.11180183453657318No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT6660.1095000320608202No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC6500.10686940065995966No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT6280.1032522824837764No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT6160.101279308933131No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC6140.10095048000802342No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTT150.002225093369.9944930
GCGATAT150.002225093369.9944928
CGATATA150.002225093369.9944935
TATTCCG207.9319376E-569.9887443
TCGTATT207.9319376E-569.9887440
AGGGGGG319050.066.8903370
CATATAC71200.065.297231
ATATACA74200.064.1092762
ACGACTA453.8562575E-1062.2122147
CAAATAC13200.061.795761
CACTTAT3700.058.6633261
AAATACA15050.058.3770942
CTCGAAA900.058.32394839
CGACTTA308.383358E-658.32394848
CTAATAC13000.058.16841
TTTATAC7150.057.277643
ATAACGT553.6379788E-1157.2635137
TAACCGA800.056.87052511
TCTTTAT6750.056.5326651
AAGGGGG377900.056.34845469
Produced by FastQC (version 0.11.5)