FastQCFastQC Report
Thu 26 May 2016
SRR1781332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723601
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33678846.543329818504944No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG40590.5609444984183272No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7440.10281909505376581No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT150.002222139270.01929548
ATGCGCG401.364242E-1069.95644
TAAGCGG150.002230073869.95644
TGCCGGG453.8744474E-1062.1963619
TTGTGCG1150.060.856882
CGCGTCT1450.060.3155757
CCGACGG308.39419E-658.31311821
TGTGCGC1100.057.241013
TGACCGT252.3863731E-456.0193153
CGGGTGT509.858923E-1055.9728557
GGACGTG252.3961761E-455.9728556
CGCGTGT509.858923E-1055.9728557
TGTGCGG750.055.9689873
ATGGACG252.3978131E-455.965124
CTGCCGG2400.055.39363518
TGCGCGT1200.055.3898055
ACGTCTG700.054.973348
TATGCGC452.7135684E-854.4142953
GTGCGCG1750.053.9663664
GCGCTGC2750.053.43232715
Produced by FastQC (version 0.11.5)