FastQCFastQC Report
Thu 26 May 2016
SRR1781332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723601
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT18526025.602507459221314No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT478686.615247905959223No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG353244.881695851719386No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC150212.075867777960506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65040.8988378954700174No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC46020.6359858540825676No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT45040.6224424786588189No Hit
CAAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT37790.5222491400647594No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT28430.3928960849971186RNA PCR Primer, Index 45 (95% over 23bp)
CACTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC20810.287589431192052No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC13730.1897454536408877No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTATCTCGTATGCCGTC13170.18200638197017416No Hit
TCATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG12310.17012137904729263No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG11370.15713079445716632No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG10810.14939172278645277No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT10670.14745695486877436No Hit
CATAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT10410.14386381445022878No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT10330.142758232782984No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT8210.11346031860099696No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC7330.10129892026130422No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGG150.002224814469.9980630
CGGAAAG150.002225424269.99322514
TGGCAAC150.002225424269.99322514
GACGGAG150.002225424269.99322537
TCGTATC150.002225424269.99322540
TCTGGCG150.002225424269.99322544
CGGTAAT1250.067.1981428
AGGGGGG297800.067.1044670
CATATAC65400.065.6324841
TACGAGA550.063.63020713
CACGTAA550.063.63020739
ATATACA69900.062.855412
GACGGTA1350.062.22050526
TAACTCG3100.062.09076736
GCTTGCA401.0661097E-861.25254456
CCAGACG401.0672011E-861.2440823
CGATCAT352.9749935E-759.9983428
ACGGTAA1400.059.9983427
AAATACA11600.059.7562452
GGTCAGT308.37834E-658.3317229
Produced by FastQC (version 0.11.5)