FastQCFastQC Report
Thu 26 May 2016
SRR1781331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399473
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24396961.07271329977245No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG32410.8113189126674393No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7270.18198977152398285No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5440.13617941638108208No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5010.12541523457154802No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4740.11865632971439873No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4440.11114643542867728No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4060.10163390266676346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG1300.064.591472
ATAGACA308.39474E-658.304444
TGTGCGC1350.057.0087853
CGTATCA5400.056.38919456
AGATTCC252.3920364E-455.9862818
TACACGT252.3935168E-455.9792675
CGCGTCT1450.055.496697
CCGTATC5300.055.47894755
GTATCAT5650.053.8873557
GCCGTAT5600.053.7570254
CGGTGGT6750.052.9090546
CGCCGTA5700.052.8205353
TCGCCGT5850.052.66304452
GTCGGCT200.006931390652.53317637
GGTCGCC6000.052.5134450
TAAGCGC200.006945142552.50685555
GTACGAC200.006945142552.50685532
CTATTGG200.00695202652.49370629
GAGCATA200.00695202652.49370631
CTCGTCA200.006955469552.48713323