FastQCFastQC Report
Thu 26 May 2016
SRR1781331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399473
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT7055217.66126872154063No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT378789.481992525151888No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT358588.976326309913311No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT219195.486979094957607No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC97482.4402149832404194No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG80932.02591914847812No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG46721.1695408700963519No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43331.0846790646676996No Hit
ACATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG25820.6463515681910917No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG25770.6450999191434715No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC19370.48488884104808083No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT18170.45484926390519503TruSeq Adapter, Index 18 (95% over 21bp)
CAAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT16180.40503363180990953No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT12750.3191705071431611No Hit
CATAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT12510.31316259171458394No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC9460.23681199980974932No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT7960.19926252838114214No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC7920.19826120914304596No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT7040.17623218590492976No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6140.15370250304776542No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT5830.14594227895251996No Hit
ACTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC5190.12992117114298088No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT4990.12491457495249993No Hit
CATATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT4590.114901382571538No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGT150.00222219970.0130264
CGTAAAT150.00222219970.0130258
TTACGGG150.00222219970.0130264
CTCGTTT207.924822E-569.9954951
TATACGA353.7071004E-969.9954911
GCCGTAA353.7071004E-969.9954946
CTTACGC150.00222440769.9954922
CCCGATG150.00222440769.9954929
TAATCCG150.00222440769.9954943
AGGGGGG235150.067.3929370
CTAATAC54200.067.356491
TAATACA55000.066.3131262
TCAACGA900.066.1068611
CCGTCTA4400.065.2230747
CAAATAC42450.064.80961
AATACAC98200.064.479483
AAATACA43450.063.8819052
CAAAAAC8950.063.7468951
CCGAGAA1500.062.9959414
CGAGAAC1400.062.49597515
Produced by FastQC (version 0.11.5)