FastQCFastQC Report
Thu 26 May 2016
SRR1781330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291573
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17907161.415494575972396No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG16750.5744702012874991No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.12072448409146251No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3330.11420810568879836No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3060.1049479890113282No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACC150.002219042370.033342
GGTGTAG207.900067E-570.033335
TTCGATT150.002219042370.033339
AAACCGA150.002220553870.02128658
AGGACGA207.906788E-570.0212855
GCGTATC207.906788E-570.0212855
GCGGTGT150.00222206670.0092733
GCTTAGG207.933719E-569.973241
CGTGTGC207.933719E-569.973241
CACCGTT150.002226607669.9732421
TATGCGG150.002228123169.961243
TATGCGC150.002228123169.961243
AGCGGGT150.002228123169.961247
ATGCGGG150.002228123169.961244
GTAGATC3000.065.3644138
CCGTATC2800.062.51900555
GCCGTAT2800.062.51900554
TGTGCGC453.8562575E-1062.1877753
AGATCTC3100.062.1263240
TGTAGAT3000.061.86274737