FastQCFastQC Report
Thu 26 May 2016
SRR1781330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291573
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT9807133.63514454356199No Hit
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT234328.036409406906674RNA PCR Primer, Index 47 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG164325.635638416451455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC63352.1726977463619743No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT27990.9599654288977374RNA PCR Primer, Index 47 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26740.9170945183538942No Hit
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT23610.8097457583521108RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC23290.798770805252887No Hit
CAAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT18180.6235145229496558No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT14170.4859846419250068Illumina PCR Primer Index 9 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT12440.4266513017323278No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT8230.2822620750206638No Hit
CACTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC7820.2682004163622832No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC7390.2534528231352011RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCG6190.21229674901311166No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT5550.1903468428146639No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5350.183487497127649No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCG5340.18314452984329824No Hit
TCATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5030.1725125440284251No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG4920.16873990390056692No Hit
CATAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT4870.16702506747881318RNA PCR Primer, Index 47 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT4360.14953373597692515No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCTCGCAGATCTCGTATGCCGT4280.1467899977021192No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC3630.12449712421932072No Hit
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3360.11523700754185058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAG150.002221225870.0159561
TCGCAAA150.002222737870.00394431
CAACGGT150.002222737870.00394412
GACACTA252.834724E-670.00394426
ATATACG207.916499E-570.00394410
GTCATAT207.923228E-569.9919449
AAGACGT207.923228E-569.9919444
ATCGTAA455.456968E-1269.9919439
CTCGAAA550.069.9919439
GTCTTAA207.923228E-569.9919449
CTCTAGG207.923228E-569.9919444
AGGACCG150.002224250569.99193620
CCGTATA150.002224250569.99193647
GTGTCAT150.002224250569.99193636
GGACCGA150.002224250569.99193621
TAGGATG150.002224250569.9919365
AGGGGGG158750.067.13862670
CATATAC34850.066.589121
ATATACA35750.066.17422
CAAATAC6100.064.265921
Produced by FastQC (version 0.11.5)