FastQCFastQC Report
Thu 26 May 2016
SRR1781329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576108
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34329859.58917425204996No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG38960.6762620897470613No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9330.1619488012664292No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7500.1301839238476119No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAG150.00221838970.0472954
AGCGGAA150.002219918970.0351134
CCCGAGT150.002220684470.0290233
TATGCGA150.002222215570.0168565
ACCCGAG207.919219E-570.0107732
TTATGCG455.456968E-1269.952
CGAATTG150.002230651769.9522
CGTAGAG150.002231419869.94392416
AGCGATG150.002231419869.9439245
TATGCGC401.364242E-1069.937863
CGCGTCT1150.060.820817
CGTCTGG1750.059.9571469
TGCCGGC2650.059.38635319
GGACGCT308.4103485E-658.29166813
CGGCTGG900.058.2916689
CGGACGC308.4103485E-658.29166812
TATGCAC308.419009E-658.2815483
CTGCCGG2850.057.67306518
GTGCCGG553.6379788E-1157.2268518
GCGAAGT252.398485E-455.96000311
Produced by FastQC (version 0.11.5)