FastQCFastQC Report
Thu 26 May 2016
SRR1781329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576108
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT18312331.786227582328312No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT488908.486256049212995No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG349166.06066918008429No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC134352.332028022523555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62231.0801794108049185No Hit
CTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT48960.8498406548772106No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC43470.7545460226207586No Hit
CAAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT40440.7019517173863234No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT26350.45737951911794317Illumina PCR Primer Index 6 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC18570.3223353954466871No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT13940.241968519791428No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC12920.2242635061481528No Hit
TCATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG12150.2108979566331313No Hit
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT11290.19597020003193846No Hit
CCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG10930.1897213716872531No Hit
CATAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT10650.1848611718636089No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATCTCGTATGCCGTCT9020.15656786574739456RNA PCR Primer, Index 41 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG8970.15569997292174384No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT8340.14476452331854445No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCAGT7030.1220257312864949No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT6870.1192484742444125No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC6770.11751268859311102No Hit
ACTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG6540.11352038159511757No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC6370.11056954598790505No Hit
CATATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT6110.10605650329452117No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT6070.10536218903400058No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGACATATCTCGTATGCCGT5770.1001548320800961No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTG150.00222498569.9948837
CGGTCGT150.00222498569.9948824
GCGTCAT150.00222498569.9948841
AGGGGGG306550.067.1526370
CATATAC69200.065.246561
ATATACA71750.064.488682
CAAATAC12400.063.791191
CACGTAA1000.062.9953939
CGTCAAC900.062.2176748
GATACAC352.9730654E-760.000823
CACAACG352.974848E-759.99561326
ACGTAAG1000.059.49564740
TAACCGA650.059.22643311
AAATACA14500.059.1387372
TGTATCG308.378442E-658.3290679
TAACTCG4700.058.0808636
AACTCGT4100.057.19093737
CGACTTC3250.057.0727548
CGAATGC4600.057.06104341
ACTCGTA4050.057.03286438
Produced by FastQC (version 0.11.5)