FastQCFastQC Report
Thu 26 May 2016
SRR1781328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859842
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33207238.62011857992515No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG35090.40809823200076295No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9660.11234622174771645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA6550.052.92245556
GGCCCGT200.006935389252.530753
ACGATCG200.006936986552.52764537
AACGACG200.00696738552.46959720
CGGTACC200.006968987652.4665510
CGCCGTA6400.051.98350553
CGTCTGG1700.051.437799
CGCGTCT1500.051.300627
CGCGTGT552.2992026E-950.8766487
TCTCGGT7450.050.2978543
CTCGGTG7950.050.2180244
TCGGTGG8250.050.08696445
TTGTGCG1200.049.569032
ATCTCGG7300.049.41819442
TGCGCGT1700.049.3774075
CCGTATC6850.049.07406255
GATCTCG7500.049.03436341
CGGTGGT8150.048.5559946
GTGGTCG7950.048.45316748
TTATGCG651.8553692E-1048.4475632
Produced by FastQC (version 0.11.5)