FastQCFastQC Report
Thu 26 May 2016
SRR1781328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859842
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT18080821.02804933929722No Hit
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT472795.498568341625554No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG339973.9538659428127496No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC143591.6699579690222157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58990.686056275455258No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT47390.5511477690087249No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC40430.47020266514080494No Hit
CAAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT38560.44845448349813105No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT25720.29912472291420983RNA PCR Primer, Index 20 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC19900.23143786881775955No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG12130.14107242958590066No Hit
TCATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG11930.13874642085406388No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC11880.1381649186711047No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT10640.12374366453371667No Hit
CATAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT10360.12048725230914517No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT9010.1047866933692469No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT8910.10362368900332852No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG8820.10257698507400197No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATT207.930223E-569.9951247
AGGGGGG292450.067.4298570
CATATAC67800.063.6128771
TACGAGA501.4551915E-1162.995613
ACTCGTA3700.062.42807438
ATATACA71950.061.4485052
AACTCGA750.060.66243437
ATGCCGA5500.057.90505244
TCGTAAG5800.057.32358640
AAGGGGG346900.056.8257669
CCGTCTA3450.056.8076347
CTACGAG3400.056.61369312
CCGACTT4150.056.5020847
TGCCGAC5350.056.2577645
CACGTAT3800.056.1802939
ATGGGGG2750.056.0123870
CGACTAC1250.055.99609448
TCGAAAG1250.055.99609440
ATCTACG3250.055.9960910
CGACTTC4500.055.9960948
Produced by FastQC (version 0.11.5)