FastQCFastQC Report
Thu 26 May 2016
SRR1781327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789340
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31873040.37930422884941No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG37200.47127980338003905No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9850.12478779740035979No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA150.00222217770.0195959
CGAACGT150.002228890869.96635425
TTATGCG301.027729E-769.961922
TATGCGG252.8504328E-669.957493
TCGACCG150.002230011269.957495
CGTATCA7100.059.66079756
CCGTATC6800.059.20398755
TCGCCGT6650.058.4411152
CGCCGTA6900.058.34966353
GTTATGC308.370067E-658.3422621
ATCTCGG7200.057.39900642
GTGGTCG7850.057.08965748
GATCTCG7400.056.32096541
GTCGCCG7250.056.01922251
GCCGTAT7250.056.01567554
GCCGTTA252.396261E-455.97308716
TCTCGGT7850.055.769343
CGGTGGT8550.055.7022346
GGTCGCC7300.055.1559150
CGCGTCT1150.054.7493447
Produced by FastQC (version 0.11.5)