FastQCFastQC Report
Thu 26 May 2016
SRR1781327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789340
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT17508822.181569412420505No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT455015.764436111181494No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG328634.163351660881243No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC127121.6104593711201765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60500.7664631210885042No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT49030.621151848379659No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC46560.5898598829401779No Hit
CAAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT38870.4924367192844655No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT28870.36574860009628296No Hit
CACTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC17230.2182836293612385No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT14610.18509134213393466No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC13800.1748296044796919No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11570.1465781539007272No Hit
TCATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11540.14619808954316266No Hit
CATAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT10890.13796336179593077No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT10800.13682316872323713No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG9490.12022702510958523No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT7960.1008437428737933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCTA150.002221835570.0223169
GCCGAAT301.0243093E-769.9956946
CCGAATT150.002225747669.9912647
AGGGGGG281950.067.4437470
ATGACGT5950.064.70190444
CATATAC68250.064.213381
TGACGTA550.063.63244645
ATATACA72900.061.7941472
TATGACG5850.061.6201443
CCGACTT4500.061.4367747
CGACTTC4400.061.24234848
TGACGTC5750.060.86581845
TCGTAAG6000.059.49633840
TCTCGAA6300.059.44078438
AAGCCGT5750.059.03984544
ATGCCGA5450.058.4367744
ACTCGTA4150.058.18919438
CCGTCTA3850.058.17455347
CACGTAT2350.058.07785439
ACGTAAG850.057.64351340
Produced by FastQC (version 0.11.5)