FastQCFastQC Report
Thu 26 May 2016
SRR1781326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468524
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34928674.55028984641129No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12930.27597305580930753No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9100.19422697663300065No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8740.18654327206290394No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8730.1863298358248457No Hit
CTTATACACATCTGACGCTGCCGACGACCCTATCGGTGTAGATCTCGGTG8290.17693864135028303No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6830.14577695059377963No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6650.14193509830873124No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6440.13745293730950814No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4790.10223595802989814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATGC150.00221841670.04539
TGCCCTG150.00221841670.04543
GAATGCC150.00221841670.04540
AATGCCC150.00221841670.04541
GTAGATG207.89853E-570.04538
GACGTAT150.002219356870.0375254
AGTATCA150.002219356870.0375256
CGCAGTA150.002219356870.0375253
GCGTATC150.002219356870.0375255
AGCAGTA252.831137E-670.0300459
GTCATTA207.906898E-570.0300359
TGTCATT207.906898E-570.0300358
TGTCAGT207.906898E-570.0300358
AGCGTGA150.002220297770.0300359
CTTCAGT150.002220297770.0300369
GATCAGT150.002220297770.0300358
TTCAGTT150.00222123970.0225570
GAGTAAA401.3460522E-1070.0075861
AATCGGT150.002223122670.0075831
TCTTGTA150.002226893369.9776841
Produced by FastQC (version 0.11.5)