FastQCFastQC Report
Thu 26 May 2016
SRR1781326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468524
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT21103245.04187618990703No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG315526.734340183213666No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT245245.234310302140339No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC132012.817571778606859No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT97822.087833280685728No Hit
ACTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG50251.0725170962426684No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC45650.974336426735877No Hit
CAAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT39550.844140321520349No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT31960.682142216834143RNA PCR Primer, Index 17 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT23200.49517207229512256No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC18040.38503897345706944No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAACAGATCTCGTATGCCGT17550.3745805977922156No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT16260.3470473230827023No Hit
TCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG14330.30585412913746146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13310.28408363285552074No Hit
CATAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT13190.28152239799882184No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG11420.24374418386251293No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTACAGATCTCGTATGCCGT10730.22901708343649418No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG10500.2241080499611546No Hit
CACTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9160.19550759406135013No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCG8860.18910450691960284No Hit
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC8200.17501771520775883No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGACGT7240.15452783635416756No Hit
CTTATACACATCTCCGAGCCAACGAGACAAATACAGATCTCGTATGCCGT7200.15367409140193458No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATAAAGATCTCGTATGCCGT7030.15004567535494448No Hit
CTAAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT6940.1481247492124203No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC6840.14599038683183785No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC6760.14428289692737192No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCAGT6580.14044104464232354No Hit
CTTATACACATCTCCGAGACCACGAGACAAATACAGATCTCGTATGCCGT6060.12934236026329493No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG6060.12934236026329493No Hit
ACTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC5730.12229896440737294No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT5490.11717649469397513No Hit
CAAAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT5150.10991966259999487No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT5140.10970622636193664No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT4800.10244939426795639RNA PCR Primer, Index 17 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG150.002219468870.0366464
GAAAAGA150.002220409270.0291560
AACGTTC150.002222290770.014257
ACGTTCC150.002222290770.014258
AAGACGG150.002222290770.014269
TCCTCCA150.002222290770.01426
AGGGGGT500.070.014270
AGGGGGA150.002222290770.014270
TAGGCGT150.002222290770.01426
CTGGGGG150.002222290770.014270
ATCGGGG207.915763E-570.014270
AGGTGTG150.002222290770.014270
AGGTGGG650.070.014270
TTCCTCC150.002222290770.01425
AATCGGG150.002222290770.014269
CCTCCAC150.00222323270.006727
TTGCACG150.00222323270.006724
GTACAGA150.002224173669.9992531
TCATTAT401.3460522E-1069.999251
CTTTTAT301.023036E-769.999251
Produced by FastQC (version 0.11.5)