FastQCFastQC Report
Thu 26 May 2016
SRR1781325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484498
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30100662.127397842715546No Hit
CTTATACACATCTGACGCTGCCGACGACCCTATCGGTGTAGATCTCGGTG15540.3207443580778455No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8130.16780255026852536No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6480.13374668213284677No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5370.11083637084157211No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5190.1071211852267708No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5000.10319960041114722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT150.002220196670.03120453
GCGGAGT150.002220196670.03120452
GCGTAGC150.002220196670.03120455
GGCACTT150.002224749469.995051
TTCGATG150.002224749469.9950530
TACGCGG207.9430014E-569.966155
GGTACGC252.8484246E-669.958923
ACGTCTG550.063.6055838
CGGGAGA401.0633812E-861.2646461
CGTCTGA352.982306E-759.9709789
ACGCGTC700.059.9709786
GTACGCG650.059.1960144
CAGGCGT252.3856116E-456.01917647
GTTCACG252.3856116E-456.01917634
TCGATGT252.3892673E-456.0018231
CCTGTTG252.3953701E-455.9729125
ATTGAAC252.3953701E-455.9729125
CGTCTGG1700.055.561359
CCGTATC2950.054.60059755
CGCGTCT1350.054.4181147