FastQCFastQC Report
Thu 26 May 2016
SRR1781325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484498
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT16414233.878777621373054No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG268795.547804118902452No Hit
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT168983.487733695495131No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC114362.360381260603759No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT73471.5164149284413972No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT59951.237363208929655No Hit
ACTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG38050.7853489591288303No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC36280.7488163005832841No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT26550.5479898781831917RNA PCR Primer, Index 43 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23800.49123009795706074No Hit
CAAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT22780.47017737947318666No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT18090.37337615428753057No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT13810.2850372963355886No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG13410.2767813283026968No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC11580.23901027455221693No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT11570.23880387535139463No Hit
CATATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT11370.23467589133494876No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG9890.20412880961324917No Hit
TCATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG9880.20392241041242687No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG9540.1969048375844689No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGACGT9310.1921576559655561No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGAATCTCGTATGCCGT9160.18906166795322168No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTAGTATGCCGT7830.16161057424385653No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAACAGCATCTCGTATGCCGT7690.1587209854323444No Hit
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT7370.15211621100603098No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC7050.14551143657971757No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCC7000.14447944057560608No Hit
CATAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT6740.13911306135422644No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCCGCATCTCGTATGCCGT6300.13003149651804546No Hit
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC5530.11413875805472883No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGA5480.11310676205061734No Hit
CTAATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT5050.10423159641525868No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGAATGCCGT5040.10402519721443637No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG4920.10154840680456885No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT207.9256744E-569.997016
TCGAAAT207.9256744E-569.9970140
TCGCAGT150.002224502969.99712
TCCGACC301.02334525E-769.99713
TTCTGAC150.002224502969.99724
AGGGGGG244050.067.47262670
CAAATCG1000.066.4971636
GCAACTC5750.064.5189835
TAACCGA600.064.1639211
AACTCGT4650.063.9757637
ACTCGTA4650.062.4704538
CAAATAC13050.061.970561
CAACTCG6100.061.9645636
CTTAAAC6000.061.849841
CATATAC39000.061.1317021
AGCAACT7000.060.9973934
TAAACAC11100.060.5379523
AACACAT16150.060.4618075
CTAATAC19750.060.4465181
ATATACA39900.060.3482932
Produced by FastQC (version 0.11.5)