FastQCFastQC Report
Thu 26 May 2016
SRR1781324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384895
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29491276.6214162304005No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8030.20862832720612118No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6190.1608230816196625No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5850.15198950363086036No Hit
CTTATACACATCTGACGCTGCCGACGACCCTATCGGTGTAGATCTCGGTG5840.15172969251354265No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5360.13925875888229258No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5310.13795970329570403No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5130.13328310318398523No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4230.10990010262539134No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3980.10340482469244859No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.10028709128463607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG207.8945566E-570.0489139
AGGTGTC252.8256472E-670.0489140
CGTGTAG207.8996454E-570.039835
CCGGATC150.002219912170.0306955
GCCGGAT150.002219912170.0306954
GGATCAG150.002219912170.0306957
GATCAGT150.002219912170.0306958
TGGCCGT150.002219912170.0306952
CATTAGA150.002219912170.0306961
GCATTAA150.002219912170.0306960
GTAGCAG252.8300183E-670.0306957
CGGATCA207.904737E-570.0306956
CGTAGCA150.002219912170.0306956
CCGTGTA150.002221057470.0215834
CTGTGTA301.02028935E-770.0215834
GTCATAA150.002222203470.0124968
AGTCATA150.002222203470.0124967
CTTATGC301.0221265E-770.003381
CTTATAA150.002223349670.003381
GGTAGAC150.002223349670.003381
Produced by FastQC (version 0.11.5)