FastQCFastQC Report
Thu 26 May 2016
SRR1781324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384895
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT17416345.24948362540433No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG273437.1040153808181445No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT187704.876654672053418No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC126153.2775172449629117No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT74631.93897036854207No Hit
ACTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG40451.050935969550137No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC38861.0096260018966212No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT30900.8028163525117239RNA PCR Primer, Index 24 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT28290.7350056508918017No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT19300.5014354564231803No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT18360.4770132113953156No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC14490.37646630899336186No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT13900.36113745307161693No Hit
TCATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG12000.311773340781252No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG11360.2951454292729186No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10490.2725418620662778No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT10150.2637082840774757No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG9770.2538354616194027No Hit
CATAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT9610.24967848374231935No Hit
CACTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC9000.23383000558593903No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC6890.1790098598319022No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACGT6280.16316138167552188No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC6270.1629015705582042No Hit
CTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCGT6230.16186232608893333No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC5870.15250912586549578No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCAGT5520.14341573675937594No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG5070.13172423648007897No Hit
CTAAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT4750.12341028072591226No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT4720.12263084737395913No Hit
CTTATACACATCTCCGAGACCACGAGACGACTTAGTATCTCGTATGCCGT4650.12081216955273516No Hit
CATATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT4640.12055235843541745No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC4540.11795424726224035No Hit
ACTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC4520.11743462502760493No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT4280.11119915821197988No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTTAGTATCTCGTATGCCGT3970.10314501357513088No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTAAGCCGT3870.10054690240195378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC252.8341474E-670.013523
TACGCAT252.8341474E-670.013525
TGATACA207.914356E-570.013512
ACGCATC301.0211079E-770.013516
TCATTAT301.0211079E-770.013511
GGCAAGC150.002222074470.013513
AAAGGCT150.002222074470.0135168
CTGATAC207.914356E-570.013511
AGGGAGG207.914356E-570.0135170
TTCGTAT150.002223220270.00442532
CCATGTT150.002223220270.00442562
ACCACTT150.002223220270.00442527
CTTAGGA252.8363374E-670.00442531
CTTAGAC301.0220356E-770.00442531
CACTTAG150.002223220270.00442529
TAACTAT150.002223220270.00442532
GACTAAC252.8363374E-670.00442529
ACTTAGG207.919452E-570.0044230
GACTTAC353.7034624E-970.0044229
CAGAATG207.924551E-569.9953254
Produced by FastQC (version 0.11.5)