FastQCFastQC Report
Thu 26 May 2016
SRR1781323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978852
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33677934.40550767633922No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10280.10502098376465492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT150.002224888469.99934466
GTACGCG352.983088E-759.9779784
GGTACGC308.3974155E-658.311923
CAACGGG252.3867501E-456.0195147
TTCGGTA252.395202E-455.97944323
GCGTCAA252.395202E-455.9794434
GTTGGCG200.00694216152.5182948
CGAAACG200.00695339552.4968231
CTACCGT200.006959017452.4860914
CGTGCAC200.00696042352.48341418
TATGCGC800.052.4807323
CTGACGG407.5021853E-752.48073212
TACGCGG200.006961829552.4807325
AGCGTCC352.0829517E-550.01741847
CGGTGGT4200.047.521446
GTGGTCG4200.047.51654448
CGCCGTA2900.047.0781453
TTGTACG305.880727E-446.663842
CGTATCA3100.046.29934756
CGTCTGG2200.046.1194279
Produced by FastQC (version 0.11.5)