FastQCFastQC Report
Thu 26 May 2016
SRR1781323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978852
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT19591020.014261604410063No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG304933.1151798228945746No Hit
CATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT207552.120340970851569No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC138841.4183962437631021No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT79310.8102348465345119No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC47060.4807672661444222No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT41450.4234552312300532RNA PCR Primer, Index 45 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG40300.41170677487505775No Hit
CAAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT28750.2937114088748861No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC21490.21954289310334965No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT18910.19318548667214247No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT17560.17939382051627825No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC16500.16856480857167377No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT15440.15773579662706924No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG12900.13178703215603585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11880.1213666621716051No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG11450.11697376109973724No Hit
TCATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG11330.11574783521921597No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT11010.11247869953782594No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC10930.11166141561747844RNA PCR Primer, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTC150.00222391770.0070570
TCGAGCT150.00222391770.0070569
GTACGGT252.840703E-669.9998926
AGGGGGG293100.065.97047470
AAACTCG7200.056.38592536
CATATAC46450.056.378881
ATTACCG252.389069E-456.00853
TACGGGC252.3914824E-455.9970616
GTATCGA252.3914824E-455.9970648
CCGTTGC252.3914824E-455.9970647
ACTCGTA5900.055.1665938
GACCACG5850.055.03984518
CCGTCAT8500.054.76183347
ATATACA48650.054.6098142
ACTCGAA1350.054.44158638
TCTCGAA7300.054.1752438
AAGGGGG358150.053.95908469
ACGTCAT1300.053.84332347
AATGCCG7250.053.10065543
CACGTAT4500.052.8861139
Produced by FastQC (version 0.11.5)