FastQCFastQC Report
Thu 26 May 2016
SRR1781322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686889
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29214142.53103485424865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCA252.8313552E-670.03499656
TACCGTC150.002226542469.9839829
ACGCGTG207.961143E-569.938126
TTGTGCG900.062.194392
AACGGTG352.9699186E-760.01249332
TGCGCAT553.6379788E-1157.22215
GTTTCGG252.3844598E-456.02799655
CGTGTAA252.3913395E-455.995341
CTTGCAT200.006939125752.52241533
GGACGAT200.00694112652.51858569
CCGTCTG200.006951136552.4994531
CGTAGAC200.0069531452.4956251
CATTACG200.00697118952.46122718
GTATCAC200.00697118952.46122716
ACCGGCG200.006973196352.4574058
TATGCGC407.52947E-752.4497683
CTGCCGG3300.050.87149418
TCTGGCG3100.050.76523211
TGTGCGG900.050.507193
GTGTCGT352.082225E-550.017757
Produced by FastQC (version 0.11.5)