FastQCFastQC Report
Thu 26 May 2016
SRR1781322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686889
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT17254425.11963359436532No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG273673.9841954085740197No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT190832.7781781335849023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC119841.7446778154840157No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT67160.9777416729631717No Hit
ACTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG41050.5976220320896097No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC34670.5047394848367058No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT32530.47358452384591976Illumina PCR Primer Index 10 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT25140.3659979996768037No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT17360.2527337022430116No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT15020.21866706265495592No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC14120.20556450896724215No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG11160.16247166572765032No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG11010.16028790677969804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10290.14980586382952704No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTAATCTCGTATGCCGT10240.1490779441802096No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG9830.14310900305580668No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT8950.13029761722781993No Hit
CACTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC8590.12505659575273442No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGACTTATCTCGTATGCCGT8270.12039790999710288No Hit
CATAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT7370.10729535630938913No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGCCTTATCTCGTATGCCGT7220.10511159736143687No Hit
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC7090.10321900627321154No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT150.00222446770.00043526
GTCGTCG150.00222575269.990248
AGGGGGG252350.067.2281370
ATGTGCC750.065.3337428
CATATAC36950.058.362231
TCCGACT308.376204E-658.333735
ATCACGA900.058.32520337
ACGTAAG601.8189894E-1258.32520340
GACGACA308.383438E-658.32520346
ACTCGAA1100.057.26474438
CCGACAA553.6379788E-1157.26474447
GCCGACA1350.057.0290946
TCGAAAG1350.057.0290940
TAAATCG1000.055.9962736
GGCGACA252.3911441E-455.9962725
ACGACAC509.822543E-1055.9962722
TCTCGAA6750.055.992238
AAATCGA252.3920044E-455.99219537
AAGGGGG306200.055.35927669
GACAACG950.055.25545518
Produced by FastQC (version 0.11.5)