FastQCFastQC Report
Thu 26 May 2016
SRR1781321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276799
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25423819.912139655497853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1650.053.0416256
CGTCTGG2500.047.572869
TTGTGCG1250.044.7867362
TCTCGGT2900.041.0609443
TCTGGCG4150.040.46043411
TTATGCG801.4297257E-939.3633422
GGCGGAT459.1887916E-538.8987154
CGGTGGT4000.038.52180546
TCGGTGG4350.037.83750545
CCGTATC2050.037.5704655
CGCCGTA2150.037.45131753
CTCGGTG3500.037.02240444
TCGCCGT2650.036.9904752
CTGCCGG4500.036.54186618
GTGGTCG4050.036.3097348
TGTGCGG1450.036.1834223
GGTCTCG1500.035.02256841
TGCCGGC4200.034.98689719
CGCGTCT1900.034.9800457
TGGCGCT5200.034.3100413
Produced by FastQC (version 0.11.5)