FastQCFastQC Report
Thu 26 May 2016
SRR1781321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276799
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT14802811.593680759461748No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG242161.8966180267998332No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT162691.274202125784873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC107510.8420276018386605No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT61450.4812817052644935No Hit
ACTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG35420.27741249797344764No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC31180.24420445191451434No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT25110.19666368786316404RNA PCR Primer, Index 32 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT22140.1734023914492414No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC17280.13533845186282256No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT15330.12006588351024712No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT13750.10769118710149367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG221800.066.0541570
TATCCCG509.840733E-1055.99511343
AAGGGGG272100.053.84770269
CCGTCTT270350.053.20416347
TATGCCG277050.053.11754643
GCCGTCT274600.053.10716646
AATCTCG279850.053.08630436
TGCCGTC276750.053.03602245
CTCGTAT278750.052.9442639
ATCTCGT277100.052.9311437
CGTCTTC274850.052.91879748
ATGCCGT278050.052.9139244
TCTCGTA278300.052.8915438
GTATGCC278850.052.82486742
CTGCTTG272500.052.79722654
CATATAC39700.052.7364541
TGCTTGA274650.052.67699455
TAATCTC276900.052.56699435
CTTAATC271850.052.55218533
CCGCAAT200.00695461352.4954254