FastQCFastQC Report
Thu 26 May 2016
SRR1781320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743328
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29442439.60889405484524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC453.8744474E-1062.188436
CGTAGCA308.360725E-658.3526956
TTGTGCG900.058.297732
CGTATCA2450.057.1618256
TTATGCG509.877112E-1055.9658242
CGCCGTA2700.055.7592453
GCGTCGG700.054.9701358
CGTCGGG900.054.4148759
TACACGT452.7133865E-854.4148755
CCGTATC2600.053.86402555
CGTCTGG1900.053.3920449
ATCTCGG2600.052.5386542
TGCCTTA200.00694195652.51742653
CGAATCT200.00694195652.51742647
CCTGTCG200.006956757552.48914729
CTCGTTC200.00696416752.4750221
CCGGTTG200.00696416752.4750222
GGTACGC200.006969728552.464433
TATGCGG200.006969728552.464433
GGTCGCC3250.051.7094650
Produced by FastQC (version 0.11.5)