FastQCFastQC Report
Thu 26 May 2016
SRR1781320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743328
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT17440923.463262516681734No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG268843.6167075638210857No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT188742.539121356924534No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC121431.633599164837057No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT70910.9539530328468724No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC42700.5744435834517199No Hit
ACTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG39060.5254746222394421No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT32250.4338596151362521RNA PCR Primer, Index 45 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT25280.34009212622153345No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT18270.24578651685393257No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT17100.23004649360712903No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC14430.19412695337724398No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTATCTCGTATGCCGTC12550.16883529209178183No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT12270.16506844892160663No Hit
TCATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG11810.1588800637134616No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG11670.15699664212837403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11090.14919389556158252No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG9170.12336411382323818No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT9100.12242240303069439No Hit
CACTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC8910.11986633087950407No Hit
CATAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT8020.1078931508028757No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC8010.10775862068965517RNA PCR Primer, Index 45 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACC150.002224636569.99966437
CCAGACG150.00222582469.9902423
AGGGGGG255150.066.9951770
GACGGTA1100.063.63177526
TAACTCG4600.061.6301436
ACCGTCA401.0670192E-861.24558327
ACTCGTA4600.060.8692738
CGGTAAT1050.059.99567828
ACTCGAA900.058.33305438
ACGTTCA308.38077E-658.3291327
ACGGTAA1200.058.3291327
CGGTCAA8450.057.56981728
TAGACGG553.6379788E-1157.26859724
AGCCGAC553.6379788E-1157.26474845
AAGCCGT3850.057.26474444
GAGACGC800.056.870924
AACTCGT5000.056.6997337
TGACGTC5250.056.6587745
TCGTAAG4450.056.62132340
AAGCCGA750.055.99219544
Produced by FastQC (version 0.11.5)