FastQCFastQC Report
Thu 26 May 2016
SRR1781319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601093
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22376637.22651902451035No Hit
CTTATACACATCTGACGCTGCCGACGACCCTATCGGTGTAGATCTCGGTG59880.9961852824770875No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6910.11495725287101995No Hit
TCTTATACACATCTGACGCTGCCGACGACCCTATCGGTGTAGATCTCGGT6550.10896816299640821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT308.393383E-658.31204225
CGTATCA9800.052.87305556
GTCCGCT200.006937589552.5245539
CGTTAGA200.006944447852.5114366
CGATCAT200.00694673552.50705763
CCTGTCG200.0069558952.4895829
GACCGTC200.006962761752.47647519
TTGTACG200.006965053352.4721072
TGTACGC200.00697193252.4590073
CCGTATC9750.052.07128555
GTAGATC10900.052.0426738
CGCCGTA10000.051.8199153
TGGTCGC10800.051.5475849
CGGTGGT11900.051.2089346
GTGGTCG11600.051.0152248
GCCGTAT10100.050.9601754
ATCTCGG11000.050.94138342
GATCTCG11000.050.94138341
TAGATCT11000.050.93289639
TCGCCGT10400.050.83683852
Produced by FastQC (version 0.11.5)