FastQCFastQC Report
Thu 26 May 2016
SRR1781319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601093
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT6565010.921770840785037No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT465127.73790411799838No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT156002.5952722789984244No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT112361.869261495309378No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG93871.5616551848050135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83651.391631577809091No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC78721.3096143192484357No Hit
ACATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG50790.8449607631431408No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG43250.7195226029915504No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT26140.4348744703398642No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT22690.37747902570816827TruSeq Adapter, Index 18 (95% over 21bp)
CAAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT17700.29446358550174434No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG16110.26801177188887576No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT14720.2448872304285693No Hit
CATAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT13460.22392541586742815No Hit
ACTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC13050.2171045079546759No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC10680.17767633294681523No Hit
ACAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9040.15039270129580615No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC8570.14257361173728525No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT7570.12593725097447483No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT7560.12577088736684672No Hit
ACATTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC7460.1241072512905657No Hit
ACTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6740.11212907154134219No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA150.00222566669.9898541
AGGGGGG210750.066.7741770
CTAATAC69900.064.604411
TAATACA70950.063.6923562
AATACAC99950.061.1119423
ACTCGTA7200.060.75507738
TCGAAAG1300.059.22218340
CACGTAT4350.058.72711639
CCGTCTA4250.058.4621147
AACTCGT7450.057.7768637
AAGGGGG246300.056.63865769
GACCACG6900.056.29618518
ACTCCGA5250.055.9918811
AACTCCG5400.055.73266210
ATGCCGA6050.055.52913744
CAACTCG8950.055.52729836
CATCACG5050.055.4421236
TGCCGAC5750.055.38327445
CGAGACC6900.055.2818415
AAATACA31350.055.1572882
Produced by FastQC (version 0.11.5)