FastQCFastQC Report
Thu 26 May 2016
SRR1781318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830862
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG753259.065885790901497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC305.900227E-446.631683
GGTACGA551.3166755E-744.52545518
TACGAGG654.887406E-737.67312220
CGTCTAG851.0640906E-732.9422531
TCTGGCG2000.031.48017111
TCGGTGG1850.030.28767845
ATGCGAA1202.3119355E-929.15357217
ATCTCGG1352.564775E-1028.53835942
TGCGAAT1352.6011548E-1028.50571618
AGATTCG500.007184802628.01947638
CGAAATG500.00719749528.00934858
TACGGTG755.603345E-527.9874314
AATAGCG500.007231428327.9823745
GTACGAG906.1944356E-627.21000119
ATTCGTT657.9251995E-426.94180540
GATCTCG1305.3660187E-926.94180541
TAGCTCG657.9279905E-426.94018436
GTTACGT700.001221306425.0173939
TAAGTCG700.001225173625.00382870
GCGAATT1401.1958036E-824.98877719
Produced by FastQC (version 0.11.5)