SRR1781318_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781318_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	830862
Sequences flagged as poor quality	0
Sequence length	76
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	31385	3.7774022641545764	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG	6172	0.7428429751270368	No Hit
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	4493	0.540763688795492	RNA PCR Primer, Index 47 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT	2863	0.34458189205909046	Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT	2099	0.25262919714705934	RNA PCR Primer, Index 47 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC	2070	0.24913884616217857	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC	1975	0.23770493776343124	No Hit
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	1419	0.1707864843981311	RNA PCR Primer, Index 47 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG	1024	0.12324549684544486	No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC	897	0.10796016667027739	RNA PCR Primer, Index 47 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	6445	0.0	59.05158	70
ACTCGCA	995	0.0	48.542713	29
GCCCGTA	30	5.876713E-4	46.669476	2
CGCCGTA	30	5.880201E-4	46.66386	19
AAGGGGG	8185	0.0	46.198723	69
CGCAGAA	360	0.0	45.694443	32
CACTCGC	1045	0.0	45.55024	28
ATGCCGA	255	0.0	45.291393	44
GCCGTCT	8050	0.0	45.21467	46
TCGTAAG	240	0.0	45.202892	40
CCGTCTT	7935	0.0	45.16426	47
TGCCGTC	8120	0.0	45.083492	45
ATCTCGT	7865	0.0	45.07404	37
GATCTCG	8065	0.0	45.041073	36
TCGCAGA	8565	0.0	44.99124	31
TATGCCG	8215	0.0	44.988163	43
ATGCCGT	8185	0.0	44.982018	44
TCTCGTA	8185	0.0	44.594505	38
CGTCTTC	8025	0.0	44.567844	48
GTATGCC	8275	0.0	44.535084	42