FastQCFastQC Report
Thu 26 May 2016
SRR1781317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704609
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33412747.420200423213444No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9630.13667154407621818No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7120.1010489505527179No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA150.0022205270.0319743
CGTAGCA252.8357445E-670.0170556
GCCGTTT150.002222397870.01704434
GCGTATC150.002222397870.01704455
AGCGCCA150.00222427770.0021369
CGATGTG207.9280806E-569.99715432
TTCGATG207.9280806E-569.99715430
AGCGAAC150.002226784469.9822512
CATCGAC150.002226784469.9822515
GGGATTC150.002226784469.9822521
TACTCGT150.002229293669.962385
CTGCCGG2900.060.32952518
CCGTATC2250.059.12550755
CGTATCA2150.058.61892756
CGGGTAG308.357609E-658.35582735
CGTGGCA308.36465E-658.34753856
CGTATCG308.36465E-658.34753856
CGCCGTA2750.057.2866853
GGTCGCC3350.056.42764350
CGACATT252.3933966E-455.98579425
Produced by FastQC (version 0.11.5)