FastQCFastQC Report
Thu 26 May 2016
SRR1781317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704609
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT18848226.749871205164848No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG281944.001368134667596No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT215273.05516960470275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC125341.7788589132412447No Hit
CTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT77641.101887713611379No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC54530.7739043923651273No Hit
ACTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG42280.6000491052484428No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT40210.5706711097928071Illumina PCR Primer Index 6 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT31100.441379545251338No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC22060.3130814394933928No Hit
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT18370.2607119693333466No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT16440.23332089144475873No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT15300.217141705541655No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG15140.21487094260788608No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12530.1778291222507802No Hit
TCATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG12300.1745649005334874No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC11120.15781802389694144No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCAGT10720.15214111656251908No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGCCATATCTCGTATGCCGT10440.14816728142842342No Hit
CCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG10340.14674805459481782No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGACATATCTCGTATGCCGT9960.1413549926271166No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCAATATCTCGTATGCCGT9850.13979384311015045No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC9610.13638769870949705No Hit
CATAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT9350.1326977089421225No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC9170.13014310064163245No Hit
CACTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC9030.12815618307458462No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT8710.12361465720704674No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG8090.1148154508386921No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATCTCGTATGCCGTCT7870.11169315180475982RNA PCR Primer, Index 41 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT7260.10303586811976571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATTC150.002221872370.02122564
GGTACGC150.002225002369.99637611
GAGGTAC150.002225002369.9963769
GTGAGTC150.002225002369.9963767
ACGAGTC150.002225002369.99637622
CGGACAA150.002225628669.9914223
CGAGTCA150.002225628669.9914223
CACGAGT150.002225628669.9914221
ATACCGT252.8431186E-669.9864544
TACCGTC252.8431186E-669.9864545
TCGTAAT207.934098E-569.9864440
AGGGGGG289300.066.44646570
ACTCGAA1500.062.9922738
GCCGACA1950.062.80834646
CTCGAAA1900.062.62389439
TCGAAAG1850.062.4203440
TCCGACC453.8562575E-1062.2145913
CGTATCA401.0679287E-861.2381441
GAGACTA352.9753755E-759.99690224
ATACGAG1050.059.99263812
Produced by FastQC (version 0.11.5)