FastQCFastQC Report
Thu 26 May 2016
SRR1781316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027709
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33587032.68143024922425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG150.00222970169.9614642
TTGTGCG1250.061.5660862
CGTATCA2350.061.0837156
CGCCGTA2600.056.55962853
TTCGATG252.3922483E-455.9936930
TACGCGG252.398008E-455.966455
ACAGCGA252.398008E-455.9664512
TCGCCGT2650.055.49246252
CCGACGG452.7139322E-854.41711421
CCGTATC2600.053.86368655
GTTCGGT200.00695626452.4915329
CGTGGTT200.00696564352.4736515
AAGTTCG200.00696832452.46854411
ATCCCCG200.00696832452.46854411
GACGCGA200.00696832452.4685445
TCTCGGT3200.051.4455443
GTGGTCG4200.050.8523648
CTCGGTG3450.050.76093344
ATCTCGG2800.050.0382142
TGTGCGG1050.049.965183
Produced by FastQC (version 0.11.5)