FastQCFastQC Report
Thu 26 May 2016
SRR1781316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027709
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT19707119.175758896730493No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG298282.902378007782359No Hit
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT215502.0968970788423573No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC141911.3808383501555401No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT81580.7938044718884432No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC48490.4718261686917211No Hit
ACTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG42800.4164603015055818No Hit
CAAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT32280.31409669468692014No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT30390.2957062748307157RNA PCR Primer, Index 20 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT18840.18332037570946638No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC16300.15860520828366784No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT15530.1511128150089179No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT14320.13933905414859654No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT12990.12639764758311933No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG12750.12406235617280767No Hit
TCATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG12330.11997559620476224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12300.11968368477847327No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG11040.10742340487433699No Hit
CATAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT10510.10226630300989871No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAT150.00222443270.0032126
GGTGCGA207.9321486E-569.99315
AGGGGGG291100.067.1786470
CGCAATG950.062.6344528
TTCCGTC601.8189894E-1258.32749645
AACTCGA1450.057.92523637
CGTCAAC553.6379788E-1157.26699448
TCTCGAA8650.056.6417338
AAGCCGA2350.056.5900844
TATGACG8850.056.5480143
ATGACGT9300.056.4459644
CATATAC44850.056.1254841
GCGACGC252.3921007E-455.99439618
ACTCGAA1500.055.99439638
GTGCGAC252.3921007E-455.99439616
TCGAAAG2200.055.67624740
ACTCGTA4000.055.11948438
CTCGAAA2300.054.77712639
AAGGGGG357700.054.6119669
TGACGTC8550.054.0296845
Produced by FastQC (version 0.11.5)