FastQCFastQC Report
Thu 26 May 2016
SRR1781315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461495
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39472327.00816629547142No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA200.00694080252.522191
TTGTGCG3150.047.761992
TCTGGCG6100.045.8817611
TGCGCGT2900.045.8374985
CGTATCA3950.045.19814356
CCGTATC4050.044.0836555
CGCGTCT2800.043.7265627
TGGCGCT8150.043.35692613
CTGGCGC7600.043.27072512
CGCCGTA4100.042.69366553
CGTCTGG4650.042.12949
TGTGCGC3650.041.2093733
TCGCCGT4250.041.18682552
ATCTCGG5950.040.0182842
TTCGATG701.9292202E-839.99910430
GTGGTCG7200.039.8697748
TGCCGGC6200.039.49901219
ATTCGAT459.211772E-538.88268729
GCCGTAT4800.038.6555554
TGGTCGC5750.038.35615549
Produced by FastQC (version 0.11.5)