FastQCFastQC Report
Thu 26 May 2016
SRR1781315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461495
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT22967615.715141002877193No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG365472.500658572215437No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT260501.7824214246371013No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC161841.1073592451565009No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT99040.6776622567986891No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC58530.40048033007297323No Hit
ACTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG54140.37044259474031727No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT47030.3217937796571319No Hit
CAAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT38270.2618551551664563No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT31540.21580641740135956No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT23940.1638048710395862No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC19180.13123548147615968No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT16860.11536132521835518No Hit
TCATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG15860.10851901648654289No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG15810.10817690104995227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15000.10263463097718432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG344950.066.53116670
CGTCATA750.055.99321448
TATGACG9000.055.6043743
CCGTCAT13400.055.10526347
TAACTCG8500.054.35193336
AAGGGGG426150.053.7801669
ACTCGTA8050.053.4754438
TGCCGTC429250.053.09897645
ATGCCGT431300.053.09813344
TATGCCG433000.053.09171743
CCGTCTT418300.053.03325347
CATATAC58700.053.00221
GCCGTCT424300.052.99263446
TCTCGTA442450.052.9421738
ATCTCGT442700.052.93598637
CTCGTAT440750.052.93197339
CGTCTTC423650.052.88394548
GTATGCC435550.052.86303742
TCGTAAG10400.052.8337540
ATGACGT9350.052.7743544