FastQCFastQC Report
Wed 25 May 2016
SRR1781314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220105
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6548329.7508007541855No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG19970.9072942459280798No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7920.3598282637831944No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4590.20853683469253312No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.16764725926262464No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3140.14265918538879171No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC150.002222508770.017
AGCGTAG150.002222508770.05
TAGATGA150.002222508770.05
CGTATCA3050.055.08196656
GCCGTAT3000.054.83333254
AGATCTC3550.054.22535340
TAGATCT3550.054.22535339
CATTAAA3300.054.09090861
CCGTATC3000.053.66666855
ATCATTA3300.053.03030459
GTTTATG200.00694460952.538
GTCAGTC200.00694460952.546
TTGACGA200.00694460952.520
TAGGGTA200.00694460952.55
CAGTACT200.00694460952.525
ATCTCGG3750.052.2666742
GTATCAT3250.051.69230757
TCTCGGT3600.051.52777543
GTAGATC3950.050.50632538
TCATTAA3650.049.86301460