FastQCFastQC Report
Wed 25 May 2016
SRR1781314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220105
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2206110.022943595102337No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT64532.931782558324436No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT42751.9422548329206515No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG37041.6828331932486766No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34441.5647077531178302No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT32011.4543058994570772No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC17820.8096135935121873No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG13810.6274278185411508No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT12030.5465573249131096No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT9420.4279775561663752No Hit
CATAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT8500.386179323504691No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT7830.3557393062402035No Hit
TCAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG5140.23352490856636604No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4830.21944072147384203No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG4590.20853683469253312No Hit
CTCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC3990.1812771177392608No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT3110.1412961995411281RNA PCR Primer, Index 48 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2590.11767111151495878No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2570.11676245428318302No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT2460.11176483950841644No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2270.10313259580654688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACA301.0191252E-770.0129549
CCGCATA207.9160585E-569.9970519
AGGGGGG34950.065.19038470
CGTCATA501.4551915E-1163.01165848
CTAAAAC8600.062.6717761
AACACAT22100.061.7620965
AAACACA22900.061.285624
AAAACAC12250.061.1402783
CTTAAAC8400.060.830771
TAAAACA9100.060.7666632
TCTACAG352.96106E-760.011150
AAACTCG1700.059.70336536
TTAAACA8800.058.463442
CGTCTTA900.058.34412848
TAAACAC11500.058.1279833
GTCTTAT800.056.88552549
GCCGTAA252.3845957E-456.0103646
CCTTATA252.3872752E-455.997641
CGCATAA252.3872752E-455.9976420
TATCGAC252.3872752E-455.9976429
Produced by FastQC (version 0.11.5)