FastQCFastQC Report
Wed 25 May 2016
SRR1781313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171512
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4739627.634218013899904No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG14240.8302626055319744No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6670.3888940715518448No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3600.2098978497131396No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2590.1510098418769532No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2510.1463454452166612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGC150.002221669370.06
CGTGCTT150.002221669370.020
CTATATG150.002221669370.032
TATCCCG150.002221669370.020
CGAAACT308.349856E-658.33333225
TCAATAA308.349856E-658.33333260
GCCGTAT2550.054.90196254
ATCATTA2350.053.61702359
TATCATT2300.053.2608758
CGTATCA2500.053.19999756
CGAAAGC200.00694200752.529
AGCTTGT200.00694200752.533
TTTCCCG200.00694200752.553
TGTGATT200.00694200752.546
CCCGTTT200.00694200752.549
GGCCACT200.00694200752.549
CCCGTAT200.00694200752.516
CTCGGTT200.00694200752.515
TTTGGAA200.00694200752.558
TCCCGTT200.00694200752.548
Produced by FastQC (version 0.11.5)