##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781313_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 171512 Sequences flagged as poor quality 0 Sequence length 76 %GC 74 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.632288119781705 32.0 27.0 32.0 13.0 32.0 2 28.232934138719155 32.0 27.0 32.0 13.0 32.0 3 27.887786277345025 32.0 27.0 32.0 13.0 32.0 4 26.962445776388822 32.0 27.0 32.0 13.0 32.0 5 27.78248169224311 32.0 27.0 32.0 13.0 32.0 6 31.58244321097066 37.0 32.0 37.0 13.0 37.0 7 31.117950930547135 37.0 32.0 37.0 13.0 37.0 8 31.628743178319883 37.0 32.0 37.0 13.0 37.0 9 31.680972759923502 37.0 32.0 37.0 13.0 37.0 10-11 31.244889570409065 37.0 32.0 37.0 13.0 37.0 12-13 31.064963384486216 37.0 27.0 37.0 13.0 37.0 14-15 32.050649517234945 37.0 32.0 37.0 13.0 37.0 16-17 32.15373851392322 37.0 32.0 37.0 13.0 37.0 18-19 31.42348057278791 37.0 29.5 37.0 13.0 37.0 20-21 31.907545827697184 37.0 32.0 37.0 13.0 37.0 22-23 31.955309249498576 37.0 32.0 37.0 13.0 37.0 24-25 31.400435538038153 37.0 32.0 37.0 13.0 37.0 26-27 31.973990158123044 37.0 32.0 37.0 13.0 37.0 28-29 31.404691799990673 37.0 32.0 37.0 13.0 37.0 30-31 31.60122032277625 37.0 32.0 37.0 13.0 37.0 32-33 31.504763515089323 37.0 32.0 37.0 13.0 37.0 34-35 31.487432366248427 37.0 32.0 37.0 13.0 37.0 36-37 32.27514109799897 37.0 32.0 37.0 13.0 37.0 38-39 32.12808433229162 37.0 32.0 37.0 13.0 37.0 40-41 31.408662367647743 37.0 29.5 37.0 13.0 37.0 42-43 31.501335183544008 37.0 32.0 37.0 13.0 37.0 44-45 31.17245149027473 37.0 29.5 37.0 13.0 37.0 46-47 31.526208078735017 37.0 29.5 37.0 13.0 37.0 48-49 32.0712340827464 37.0 32.0 37.0 13.0 37.0 50-51 32.28423958673446 37.0 32.0 37.0 13.0 37.0 52-53 31.33667906618779 37.0 29.5 37.0 13.0 37.0 54-55 31.443829003218433 37.0 29.5 37.0 13.0 37.0 56-57 31.2324618685573 37.0 29.5 37.0 13.0 37.0 58-59 30.761427771817715 37.0 27.0 37.0 13.0 37.0 60-61 30.537487172909184 37.0 27.0 37.0 13.0 37.0 62-63 30.13411598022296 37.0 27.0 37.0 10.5 37.0 64-65 29.91669970614301 37.0 24.5 37.0 10.5 37.0 66-67 30.016914268389385 37.0 27.0 37.0 8.0 37.0 68-69 30.383296795559495 37.0 27.0 37.0 13.0 37.0 70-71 30.49960644153179 37.0 27.0 37.0 13.0 37.0 72-73 30.98284085078595 37.0 27.0 37.0 13.0 37.0 74-75 31.391197117402864 37.0 32.0 37.0 13.0 37.0 76 31.522068426699008 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.9594998823660994 11101 2 -0.05178836746335236 11101 3 -0.9940242336229339 11101 4 -0.18852488320993288 11101 5 -0.11293886770436146 11101 6 -0.02363233562101641 11101 7 1.2007478316934765 11101 8 -1.341577093229855 11101 9 0.781950777416494 11101 10-11 0.5972286653091352 11101 12-13 0.477336004478893 11101 14-15 -0.9139077841156009 11101 16-17 -0.07806213887210589 11101 18-19 -0.4130807491814572 11101 20-21 0.6747896229739467 11101 22-23 -0.7907579279457089 11101 24-25 0.4422028293173206 11101 26-27 -0.49375052416333887 11101 28-29 -0.012585846474856055 11101 30-31 0.18401265598579997 11101 32-33 1.36707679266123 11101 34-35 0.5221107994854819 11101 36-37 0.9481778543093213 11101 38-39 0.8636222929790947 11101 40-41 -0.23006776244637805 11101 42-43 1.1539551081879864 11101 44-45 -0.7219688399396809 11101 46-47 -0.43382747113042086 11101 48-49 0.17626117180464718 11101 50-51 0.7732247921467845 11101 52-53 0.6321710638325193 11101 54-55 0.6476161864853012 11101 56-57 -0.2576526616630552 11101 58-59 -0.7798624508184915 11101 60-61 -0.8962131292212021 11101 62-63 1.3921216514319497 11101 64-65 0.6324060884545304 11101 66-67 0.22480190916532194 11101 68-69 -0.20380873526216092 11101 70-71 0.19620258719814387 11101 72-73 -1.032324423819336 11101 74-75 -0.9020075045803893 11101 76 -1.1247277441556278 11102 1 -0.8272418312048835 11102 2 -0.8582298934170716 11102 3 -0.5390523762120516 11102 4 -1.3139157525032417 11102 5 -2.294302219799423 11102 6 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11102 66-67 -1.3369554391773946 11102 68-69 0.05885542984103154 11102 70-71 -1.784566643571086 11102 72-73 -1.2596540048074516 11102 74-75 -0.6221450905716353 11102 76 -0.6019009774264212 11103 1 0.15845524798813315 11103 2 0.8513455356705535 11103 3 1.0952445223124876 11103 4 0.12676476541304993 11103 5 0.3419519205197581 11103 6 -0.26769691301892706 11103 7 -0.48206318445087604 11103 8 -0.16351441516717813 11103 9 1.973308802107855 11103 10-11 0.49300264441644615 11103 12-13 -0.2954560183131285 11103 14-15 0.3472508103763303 11103 16-17 -0.5963432404865401 11103 18-19 0.20087936477865753 11103 20-21 -0.9458181643005119 11103 22-23 0.8832686627475503 11103 24-25 -0.3892311687833434 11103 26-27 0.30302060594112135 11103 28-29 0.16690133301232635 11103 30-31 -1.6920557200825783 11103 32-33 -0.1908529319351615 11103 34-35 -0.7540050599637098 11103 36-37 0.19081793028273353 11103 38-39 -0.6463492169924088 11103 40-41 -0.5453574110693609 11103 42-43 -0.43009047585759674 11103 44-45 0.4235677222635488 11103 46-47 -1.647740101709715 11103 48-49 0.17293733514748055 11103 50-51 0.11842897068429892 11103 52-53 -0.15439113939634908 11103 54-55 -1.1983002427644607 11103 56-57 -2.027833098510154 11103 58-59 -0.25469625898563564 11103 60-61 0.5631885801805083 11103 62-63 -2.16390873793177 11103 64-65 -1.1888189827705418 11103 66-67 0.04056639159647091 11103 68-69 -0.13400816546159078 11103 70-71 -0.8370357793735543 11103 72-73 -1.8314697229646342 11103 74-75 -0.38396381987003636 11103 76 -1.6660382854661684 11104 1 -1.3316265941536365 11104 2 -0.7158621214247454 11104 3 -0.06316857243019314 11104 4 -2.0876893891160115 11104 5 -1.2578538777879125 11104 6 -2.8043871613196636 11104 7 -2.122790053642163 11104 8 -1.4777050102566491 11104 9 -1.8079368630329427 11104 10-11 -1.2505817639619679 11104 12-13 -1.4648276371229478 11104 14-15 0.010033443196412861 11104 16-17 -0.5491799723494921 11104 18-19 -1.3979554250673694 11104 20-21 -0.8531562139606557 11104 22-23 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64-65 -0.7870476462165961 11105 66-67 0.2631240942266331 11105 68-69 0.3329805623967026 11105 70-71 -0.029131860962390732 11105 72-73 0.383506122445997 11105 74-75 0.23734589564257647 11105 76 -0.5046051131634322 11106 1 0.6796093609025462 11106 2 0.13807046284491875 11106 3 0.004247157906885235 11106 4 -0.4809478160823062 11106 5 -0.5949325294733043 11106 6 -0.523488284915171 11106 7 -1.1227900536421629 11106 8 0.21892420322649642 11106 9 0.45049010325918815 11106 10-11 0.5022272248020769 11106 12-13 0.8891049471467127 11106 14-15 -1.6585058826462813 11106 16-17 -1.279517051001175 11106 18-19 -0.7237981216965821 11106 20-21 0.13560783098316165 11106 22-23 -1.0489688181790733 11106 24-25 -0.22187092464407243 11106 26-27 -1.1269974270731637 11106 28-29 -0.5128739478865505 11106 30-31 -0.6533540971112899 11106 32-33 -0.6146287676960185 11106 34-35 -0.6127926865415994 11106 36-37 -1.2102779221239857 11106 38-39 -0.7913762665138435 11106 40-41 -1.5612403351919824 11106 42-43 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21611 68-69 -0.36681239826582157 21611 70-71 1.0567031976987558 21611 72-73 0.22408583259092296 21611 74-75 0.02564817307528955 21611 76 -0.43058855001643437 21612 1 1.3958108831932314 21612 2 -0.05964610112753732 21612 3 -0.4977463179901811 21612 4 -0.5493602843679142 21612 5 0.5400801562824036 21612 6 0.06404342947410058 21612 7 0.15194716998958668 21612 8 0.16772811768825946 21612 9 0.523886261287462 21612 10-11 0.8436099721743133 21612 12-13 0.6993169804921244 21612 14-15 1.1239334069514015 21612 16-17 0.43641263366718164 21612 18-19 0.36948748903193973 21612 20-21 0.18585248484325945 21612 22-23 0.23188475195503244 21612 24-25 0.1641838053305591 21612 26-27 -0.7470590436008919 21612 28-29 -0.9661631831167057 21612 30-31 0.5126933838600749 21612 32-33 0.6309312178059727 21612 34-35 0.41375678790566184 21612 36-37 0.19032012064513282 21612 38-39 0.851111953855856 21612 40-41 -1.2240710709658131 21612 42-43 -0.30747996211805173 21612 44-45 0.4346688771819096 21612 46-47 0.3563917182823175 21612 48-49 -0.8866006975088325 21612 50-51 0.5829849244875689 21612 52-53 0.03074426482507775 21612 54-55 0.4234639945911738 21612 56-57 0.7162927477662286 21612 58-59 0.3942483845826672 21612 60-61 0.4269878633346309 21612 62-63 -0.7940859581627535 21612 64-65 0.30341518688298663 21612 66-67 -1.1863850221829004 21612 68-69 -0.48451593129193427 21612 70-71 0.19930432417084987 21612 72-73 -0.2611292211725207 21612 74-75 0.008559079373291922 21612 76 -0.5358112842714249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 8.0 12 43.0 13 120.0 14 265.0 15 539.0 16 837.0 17 1196.0 18 1610.0 19 1912.0 20 2285.0 21 3028.0 22 3628.0 23 4316.0 24 5117.0 25 5426.0 26 5854.0 27 6485.0 28 6854.0 29 7782.0 30 8765.0 31 10187.0 32 12175.0 33 15578.0 34 20578.0 35 24373.0 36 22551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 63.3051914734829 10.129903446989132 5.35239516768506 21.212509911842904 2 51.33168524651336 12.714562246373431 18.482671766407016 17.471080740706192 3 50.32126031997761 12.130346564671859 16.847800736974673 20.700592378375855 4 49.552800970194504 11.380544801529922 13.968701898409442 25.09795232986613 5 49.797681794859834 17.981832175008165 18.185899528895938 14.034586501236065 6 61.86330985587014 17.215121973972668 7.75864079481319 13.162927375344 7 57.90032184336956 14.72316805821167 8.126545081393722 19.249965017025048 8 56.31734222678296 18.87331498670647 9.32412892392369 15.485213862586875 9 51.86692476328187 9.692616260086757 8.771980969261627 29.668478007369746 10-11 57.28607910816736 13.315686365968563 12.042889127291385 17.355345398572695 12-13 55.257649610522876 11.788387984514204 12.294183497364616 20.6597789075983 14-15 56.5024604692383 11.56595456877653 9.859368440692197 22.07221652129297 16-17 57.11728625402305 12.400590046177527 9.761416110826064 20.720707588973365 18-19 55.17398199542889 12.723891039694015 11.139745323942348 20.962381640934748 20-21 56.891354540790154 12.139092308409907 10.384113064975045 20.5854400858249 22-23 57.072682960959 12.109939829283082 9.701070479033538 21.11630673072438 24-25 56.671544848173895 12.548976164933064 9.55414198423434 21.225337002658705 26-27 57.25780120341434 12.571423573860722 10.515882270628294 19.65489295209665 28-29 56.45960632492187 13.334343952609732 11.486368300760297 18.719681421708103 30-31 56.779700545734414 12.047845048742944 11.103013200242549 20.069441205280096 32-33 57.34759083912496 12.420996781566304 10.92430850319511 19.307103876113622 34-35 56.96190354027707 12.470555996081908 10.949379635244181 19.618160828396846 36-37 57.24759783571996 11.511730957600633 12.041431503335044 19.19923970334437 38-39 56.37156583795886 11.881675917720044 13.394981109193527 18.35177713512757 40-41 56.14592564951724 12.595620131535986 12.324502075656515 18.933952143290263 42-43 55.10255842156817 12.477261066281077 13.393815010028455 19.0263655021223 44-45 54.09621484211017 12.197688791454825 14.094932133028593 19.61116423340641 46-47 54.9288096459723 12.467932272960493 13.070805541303232 19.53245253976398 48-49 54.950965530108675 12.50174914874761 13.82089882923644 18.72638649190727 50-51 55.03055179812492 12.702318205140164 13.395272633984796 18.871857362750127 52-53 53.29860301320024 13.5742688558235 13.75909557348757 19.368032557488686 54-55 54.13440458976632 12.830006063715658 12.775490927748494 20.26009841876953 56-57 54.237312841084005 13.258839031671254 12.439945892998741 20.063902234246 58-59 53.735307150520086 14.087935538038154 13.41218107187835 18.764576239563414 60-61 53.59129390363356 13.16467652409161 13.140188441625074 20.103841130649748 62-63 53.420052999670574 14.131496322382594 13.337375845793698 19.111074832153133 64-65 53.57759223844396 14.40365688698167 13.261754279583936 18.75699659499044 66-67 53.655137832921305 15.456935957833853 11.666530621764075 19.22139558748076 68-69 53.484012780446854 15.641471150706657 11.87876066980736 18.995755399039137 70-71 53.74930034050096 15.535939176267549 12.086326321190354 18.62843416204114 72-73 54.688010168384714 14.364301040160454 12.208766733523019 18.738922057931806 74-75 55.61680815336536 13.75763794953123 12.299722468398713 18.325831428704696 76 57.797122067260595 11.872638649190726 11.959513036988666 18.370726246560007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.5 10 1.5 11 4.0 12 7.0 13 7.5 14 8.0 15 8.5 16 18.0 17 27.0 18 31.5 19 44.5 20 56.5 21 60.0 22 78.5 23 117.5 24 165.0 25 192.0 26 219.5 27 283.0 28 395.0 29 471.0 30 512.5 31 599.0 32 738.0 33 832.0 34 863.5 35 990.5 36 1188.0 37 1290.0 38 1370.5 39 1529.0 40 1716.0 41 1867.5 42 1910.0 43 2019.0 44 2278.5 45 2752.0 46 3075.0 47 2811.5 48 2304.5 49 2029.5 50 1998.0 51 1963.5 52 1913.0 53 2016.5 54 2136.0 55 2211.5 56 2392.0 57 2670.5 58 2844.0 59 3119.0 60 3597.5 61 3946.5 62 4092.0 63 4258.5 64 4371.0 65 4280.0 66 4258.5 67 4274.0 68 4159.0 69 3964.0 70 3906.5 71 3929.0 72 3927.5 73 3880.0 74 3650.5 75 3467.0 76 3328.5 77 3012.0 78 2596.5 79 2359.0 80 2101.5 81 1693.0 82 1312.0 83 1082.0 84 991.5 85 784.0 86 630.0 87 593.0 88 571.5 89 542.0 90 578.5 91 647.0 92 671.0 93 785.0 94 997.5 95 1352.0 96 1608.0 97 2024.0 98 3586.0 99 27423.5 100 50115.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 8.745743738047484E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 171512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.67918279770512 #Duplication Level Percentage of deduplicated Percentage of total 1 82.68536486303533 42.73112085451747 2 8.92526738571235 9.225010494892485 3 4.529762173383276 7.02283222165213 4 2.098470147569836 4.337888894071551 5 0.9488244054334581 2.451723494565978 6 0.3858477368112279 1.196417743364896 7 0.14215442935150502 0.514249731797192 8 0.06317974637844669 0.2612062129763515 9 0.030461663432465362 0.14168104855636923 >10 0.16133399521639064 1.7538131442697886 >50 0.016923146351369645 0.5766360371285975 >100 0.009025678054063811 0.9340454312234712 >500 0.0011282097567579764 0.3888940715518448 >1k 0.0011282097567579764 0.8302626055319744 >5k 0.0 0.0 >10k+ 0.0011282097567579764 27.634218013899904 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 47396 27.634218013899904 No Hit CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG 1424 0.8302626055319744 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 667 0.3888940715518448 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 360 0.2098978497131396 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 259 0.1510098418769532 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 251 0.1463454452166612 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0011660991650729979 0.0 2 0.0 0.0 0.0 0.0011660991650729979 0.0 3 0.0 0.0 0.0 0.0011660991650729979 0.0 4 0.0 0.0 0.0 0.0029152479126824944 0.0 5 0.0 0.0 0.0 0.012827090815802977 0.0 6 0.0 0.0 0.0 0.013410140398339475 0.0 7 0.0 0.0 0.0 0.013410140398339475 0.0 8 0.0 0.0 0.0 0.013410140398339475 0.0 9 0.0 0.0 0.0 0.018074537058631466 0.0 10 0.0 0.0 0.0 0.018657586641167966 0.0 11 0.0 0.0 0.0 0.02040673538877746 0.0 12 0.0 0.0 0.0 0.025071132049069453 0.0 13 0.0 0.0 0.0 0.02623723121414245 0.0 14 0.0 0.0 0.0 0.027986379961751948 0.0 15 0.0 0.0 0.0 0.032067727039507436 0.0 16 0.0 0.0 0.0 0.03323382620458044 0.0 17 0.0 0.0 0.0 0.03323382620458044 0.0 18 0.0 0.0 0.0 0.03498297495218993 0.0 19 0.0 0.0 0.0 0.04197956994262792 0.0 20 0.0 0.0 0.0 0.0530575120108214 0.0 21 0.0 0.0 0.0 0.0542236111758944 0.0 22 0.0 0.0 0.0 0.06763375157423387 0.0 23 0.0 0.0 0.0 0.07346424739959886 0.0 24 0.0 0.0 0.0 0.08162694155510984 0.0 25 0.0 0.0 0.0 0.08920658612808434 0.0 26 0.0 0.0 0.0 0.09562013153598582 0.0 27 0.0 0.0 0.0 0.17666402350855917 0.0 28 0.0 0.0 0.0 0.2174774942861141 0.0 29 0.0 0.0 0.0 0.28744344419049395 0.0 30 0.0 0.0 0.0 0.3673212369979943 0.0 31 0.0 0.0 0.0 0.44953122813564067 0.0 32 0.0 0.0 0.0 0.5632258967302579 0.0 33 0.0 0.0 0.0 0.6914968048882877 0.0 34 0.0 0.0 0.0 0.9328793320583982 0.0 35 0.0 0.0 0.0 1.2383973133075237 0.0 36 0.0 0.0 0.0 1.658193012733803 0.0 37 0.0 0.0 0.0 2.2464900415131304 0.0 38 0.0 0.0 0.0 2.8307057232147024 0.0 39 0.0 0.0 0.0 3.4085078595083727 0.0 40 0.0 0.0 0.0 3.993306590792481 0.0 41 0.0 0.0 0.0 4.445753066840804 0.0 42 0.0 0.0 0.0 5.0218060543868654 0.0 43 0.0 0.0 0.0 5.464340687532068 0.0 44 0.0 0.0 0.0 6.00016325388311 0.0 45 0.0 0.0 0.0 6.508582489854938 0.0 46 0.0 0.0 0.0 7.028662717477494 0.0 47 0.0 0.0 0.0 7.49568543308923 0.0 48 0.0 0.0 0.0 7.925976025001166 0.0 49 0.0 0.0 0.0 8.31020569989272 0.0 50 0.0 0.0 0.0 8.678109986473249 0.0 51 0.0 0.0 0.0 9.075166752180605 0.0 52 0.0 0.0 0.0 9.452982881664257 0.0 53 0.0 0.0 0.0 9.783571994962452 0.0 54 0.0 0.0 0.0 10.129903446989132 0.0 55 0.0 0.0 0.0 10.460492560287326 0.0 56 0.0 0.0 0.0 10.770091888614207 0.0 57 0.0 0.0 0.0 11.167148654321563 0.0 58 0.0 0.0 0.0 11.46916833807547 0.0 59 0.0 0.0 0.0 11.772937170576986 0.0 60 0.0 0.0 0.0 12.081370399738795 0.0 61 0.0 0.0 0.0 12.362400298521386 0.0 62 0.0 0.0 0.0 12.667918279770511 0.0 63 0.0 0.0 0.0 12.96469051728159 0.0 64 0.0 0.0 0.0 13.316269415551098 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCGC 15 0.0022216693 70.0 6 CGTGCTT 15 0.0022216693 70.0 20 CTATATG 15 0.0022216693 70.0 32 TATCCCG 15 0.0022216693 70.0 20 CGAAACT 30 8.349856E-6 58.333332 25 TCAATAA 30 8.349856E-6 58.333332 60 GCCGTAT 255 0.0 54.901962 54 ATCATTA 235 0.0 53.617023 59 TATCATT 230 0.0 53.26087 58 CGTATCA 250 0.0 53.199997 56 CGAAAGC 20 0.006942007 52.5 29 AGCTTGT 20 0.006942007 52.5 33 TTTCCCG 20 0.006942007 52.5 53 TGTGATT 20 0.006942007 52.5 46 CCCGTTT 20 0.006942007 52.5 49 GGCCACT 20 0.006942007 52.5 49 CCCGTAT 20 0.006942007 52.5 16 CTCGGTT 20 0.006942007 52.5 15 TTTGGAA 20 0.006942007 52.5 58 TCCCGTT 20 0.006942007 52.5 48 >>END_MODULE