FastQCFastQC Report
Wed 25 May 2016
SRR1781313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171512
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT145038.455968095526844No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT46852.7315872941834973No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT29191.7019217314240405No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26671.5549932366248427No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG24811.446546014273054No Hit
CTAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT23291.3579224777275058No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC13590.7923643826671021No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG10160.5923783758570829No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT8620.502588740146462No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT7890.4600261206212976No Hit
CATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT6960.4058025094454032No Hit
CAAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT6360.3708195344932133No Hit
TCAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG3900.2273893371892346No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC3450.20115210597509212No Hit
TCTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG3400.19823685806240962No Hit
CTCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2760.1609216847800737No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT2530.14751154438173422No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2490.14517934605158822TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2120.12360651149773776No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT2040.11894211483744578No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT1990.11602686692476329No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1870.1090302719343253No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT1860.1084472223517888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA252.813982E-670.0697648
TTCCGAC150.002212905870.0697645
CCGAAAT150.002212905870.0697647
TAAGACG252.8188679E-670.0493243
ATCTGAA150.002218035570.02888552
CTGTCGT150.002223174369.9880511
CGGGATA150.002223174369.9880566
AAGTAAC207.916121E-569.98804533
TGCTTTC207.916121E-569.98804556
CCGTAAT700.065.0647847
AACACAT16950.064.620235
AAACACA17950.064.5293664
AAAACAC10400.063.9313853
AAACTCG550.063.625536
TCGAATG2100.063.32251740
TAAGCTT501.4551915E-1163.0259953
AGGGGGG25150.062.89184670
CTTAAAC5700.062.6208881
TTAAACA5700.062.6208882
TGCCGTA1350.062.28423345
Produced by FastQC (version 0.11.5)