FastQCFastQC Report
Wed 25 May 2016
SRR1781312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167294
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5445032.54749124296149No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG13470.8051693425944744No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7400.44233505086853087No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5070.30305928485181777No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3270.19546427247839135No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.16199026862888088No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1930.11536576326706277No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1810.10819276244216768No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1760.1052040120984614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA150.002221836269.997947
TTGTGCG352.9550029E-760.0161482
GCCGTAT2850.058.9456154
GTCCGCA308.350435E-658.3315934
CCGTATC2900.056.72244355
CGTATCA2850.056.48954456
GTATCAT2850.056.48954457
TAGAAGG252.381943E-456.015074
AGATCTC2900.054.30872340
ATCATTA2850.054.03347459
GTAGATC3000.053.66506238
TAGATCT3000.053.66506239
CGCCGTA3150.053.33173853
CAGCGAA200.00693432152.514134
CACGTCT200.00693432152.514137
CAAGAAA200.006942528352.4984357
CCTATGG200.006942528352.498438
TGTAGAT3150.052.2206637
TATCATT2950.052.20182858
TCATTAA2900.051.89560