FastQCFastQC Report
Wed 25 May 2016
SRR1781312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167294
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1718810.274128181524741No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT50603.024615347830765No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT36612.1883630016617452No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG30261.8087917080110465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26981.612729685463914No Hit
CTAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT25951.5511614283835642No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC11950.7143113321458031No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT11220.6706755771276914No Hit
TCATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG10940.6539385752029362No Hit
CAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT9390.5612873145480411No Hit
CATAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT8600.5140650591174818No Hit
CAAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT7890.4716248042368525No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4670.27914928210216744No Hit
TCTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG3980.23790452735902062No Hit
TCAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG3950.23611127715279684No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT3070.18350927110356618RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGAAGATCTCGTATGCCGT3000.17932502062237737No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2830.169163269453776No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTAGATCTCGTATGCCGT2420.1446555166353844No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT2280.1362870156730068No Hit
CTCATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2210.13210276519181802No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT1880.11237701292335649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACA252.814466E-670.0664546
ACGACTA252.814466E-670.0664547
CGTCTAA401.3278623E-1070.06644448
TACCGTC150.002213224570.06644445
CGAATGA353.6689016E-970.0454941
TTCGGAG150.002215854170.0454853
ACGTAAG207.906841E-570.00358640
AGAGACC207.918566E-569.98266615
TAGACAA150.002223756469.9826624
ATCAACG301.0206895E-769.9826610
GTAGCTA150.002223756469.9826633
GGATTTG150.002223756469.9826622
TAAAACA6700.064.2378162
GTCTAAT550.063.6777149
CTAAAAC6600.063.6205981
AGGGGGG28600.063.62059870
AAAACAC11050.062.6993983
CTTAAAC6950.062.430581
AACACAT19550.061.928395
AAACACA20950.061.4644854
Produced by FastQC (version 0.11.5)