FastQCFastQC Report
Wed 25 May 2016
SRR1781311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238933
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5531423.150422921906976No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG15580.6520656418326476No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7010.2933876860877317No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4320.18080382366604864No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2950.12346557403121376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC150.002222741770.0000147
ACAGGTT252.3871919E-455.99999619
GCCGTAT2950.055.7627154
CGTATCA2850.055.26315756
GTATCAT2900.054.31034557
CCGTATC3050.052.78688455
GCGGTAT200.006945332552.554
GTCCAAA200.006945332552.545
TTTTCGT200.006945332552.520
TGGTAGC200.006945332552.549
CTAGCTA200.006945332552.515
CGCCGTA3100.049.67741853
ATCATTA3050.049.3442659
AGATCTC3050.048.1967240
TCATTAA3200.047.0312560
CCACCTA305.870004E-446.66666841
TAGATCT3100.046.2903239
TATCATT3200.045.937558
TCTCGGT3300.045.60606443
TGTAGAT3950.045.18987737
Produced by FastQC (version 0.11.5)