FastQCFastQC Report
Wed 25 May 2016
SRR1781311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238933
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT176607.391193347088933No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT54012.2604663231952054No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT32971.3798847375624128No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG29391.230051939246567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29241.223774028702607No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT25281.0580371903420625No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14090.5897050637626448No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG12290.5143701372351245No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT10730.4490798675779403No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT8850.37039672209364133No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7820.3272884030251158No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7120.29799148715330237No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4430.1854076247316193No Hit
TCTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG4020.1682480025781286No Hit
TCAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG3960.1657368383605446No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT3590.1502513256854432Illumina PCR Primer Index 9 (95% over 22bp)
CTCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2810.11760619085685108No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2760.1155135540088644No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT2700.1130023897912804No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCG150.002224191369.9884936
CTAGTGT150.002226038869.9738530
GCTTACG150.002226038869.9738533
GTATAGG150.002226038869.973851
ACACGTT252.8404047E-669.9738425
CTAAAAC7250.064.6654741
AGGGGGG30900.062.3876870
TAAAACA7800.061.451382
CTTAAAC6450.060.2100451
AACACAT19750.060.0534935
AAAACAC11850.059.6401523
TTAAACA6600.059.3717422
AAACACA21250.058.7780234
CGACTTA308.29557E-658.40933248
CTCGAAA308.368021E-658.3237439
CACGTTA308.3784125E-658.31153526
TAAACAC9850.056.8315433
ACGTCTA252.3718773E-456.07295647
CGTAAAA252.3916044E-455.9790728
CGATAAC4150.055.6418528
Produced by FastQC (version 0.11.5)