FastQCFastQC Report
Wed 25 May 2016
SRR1781310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185332
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4821826.017093648155743No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG14340.7737465737163577No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5540.2989230138346319No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3630.19586471845121187No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2550.1375909179202728No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1970.10629572874624998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC150.002221952470.04
CAAGTAT150.002221952470.030
GTTCGCT150.002221952470.038
GTATATA150.002221952470.01
TTGTACG200.00694288352.5000042
CGTAGGT200.00694288352.5000043
GTGTAGC200.00694288352.5000042
CCGAAAC200.00694288352.50000418
GGTCTAC200.00694288352.5000041
GGATTGT200.00694288352.50000468
GGCCTTA200.00694288352.50000415
TCTATAG200.00694288352.5000042
GCCGTAT2550.049.41176654
CGTATCA2500.049.056
CCGTATC2500.049.055
GTATCAT2750.048.36363657
TATCATT2650.047.54717358
TGGTCGC3100.047.41935349
CGCCGTA2800.046.2553
TAGATCT2750.045.81818439
Produced by FastQC (version 0.11.5)