FastQCFastQC Report
Wed 25 May 2016
SRR1781310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185332
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT155568.39358556536378No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT48922.6395873351606847No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT31361.6920985043057861No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG27131.463859452226275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24471.3203332398074805No Hit
CTAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT23601.2733904560464464No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC12680.6841775840113958No Hit
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG10460.5643925495866876No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT8720.47050698206461916No Hit
CAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT7480.4036000258994669No Hit
CATAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT6620.357196814365571No Hit
CAAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT6300.33993050309714457No Hit
TCAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG4090.2206850408995748No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3650.19694386290548854No Hit
TCTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG3480.187771135044137No Hit
CTCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2780.15000107914445426No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT2590.1397492068288261RNA PCR Primer, Index 42 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2520.13597220123885784RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT2230.12032460665184641No Hit
CTCTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1910.10305829538342001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT252.8332797E-669.9929934
TAACGTA207.9150355E-569.9929838
TACGAGA207.9150355E-569.9929813
AGGGGGG25400.063.7928270
AACACAT14500.062.0282675
CTAAAAC6050.060.7377131
AAACACA15750.060.6605874
AAAACAC8750.060.3939483
TCGAAAG352.964316E-759.99398840
CTTAAAC5350.058.2184641
TCGTAAG850.057.64128540
TAAACAC7600.057.5600173
CAAAAAC3000.057.160941
GTAAGCC1050.056.69158642
TAAGCCG1050.056.69158643
GATTGAA1300.056.53279556
CGATATC2050.056.33581535
GATAACG252.3869703E-455.9943936
ACTCGAA1950.055.6354532
TTAAACA5700.055.2576182
Produced by FastQC (version 0.11.5)