FastQCFastQC Report
Wed 25 May 2016
SRR1781309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147767
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8683858.76684239376857No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG26231.7750918676023741No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9070.6138041646646409No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7900.5346254576461592No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4930.33363335521462845No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3040.20572929003092708No Hit
TCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGT2940.19896187917464656No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2920.19760839700339047No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2900.19625491483213436No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2290.15497370860882337No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2200.1488830388381709No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2040.13805518146812212No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2020.136701699296866No Hit
CTCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGG1670.11301576129988428No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACAAA207.905805E-570.0000166
GTAGATA150.002221058870.038
ATCCAGT150.002221058870.06
GCCTACA150.002221058870.028
AGTATCA150.002221058870.056
TCAGCGT150.002221058870.028
GCAGTAT150.002221058870.054
TGAAGCT150.002221058870.013
ATCAATA150.002221058870.059
GACTCAT150.002221058870.055
GTGCTTG150.002221058870.030
TGTGTAG401.0586518E-861.25000435
GTTGTGG308.344154E-658.3333321
GGTGGTA252.3841996E-456.00000447
AGCCGAC252.3841996E-456.00000419
AGATCTC4750.056.00000440
CGCGTGT252.3841996E-456.0000047
TAGATCT4750.056.00000439
CGTATCA4550.055.38461756
GCCGTAT4550.055.38461754
Produced by FastQC (version 0.11.5)